Pluripotent stem cells from the hair follicle (hfPSCs) after specific transdifferentiation regenerate healthy breast tissue and blood vessels after mastectomy in treated with chemotherapy,Abs,and radiation hormone refractory breast cancer animal models circumventing capsular contracture,CTD,hematoma/seroma, calcification, atrophy, necrosis,fibromyalgia syndrome,neuroendocrine immunity,diffuse systemic inflammatory disease,and teratomas.

Giannios J.,Dept of Translational Cancer Medicine,Erasinio Hospital,Alexandropulos N.,Dept of Biopathology and Genetics,Ip.Hospital,Kononas T.,Dept.of Plastic Surgery,Ippokration Hospital,Athens,Greece.

Background:We aim to circumvent complications caused by reconstructive surgery after mastectomy in breast cancer subjects with the use of autologous pluripotent stem cells from the hair follicle(hfPSCs).

Methods: We have induced orthotopically hormone refractory breast cancer in female animal models which had mastectomy after curative treatment with antiHer2/neu Abs,docetaxel and radiation.Hair follicle pluripotent stem cells were obtained from these animals,they were transdifferentiated in specific proteins,and they were injected subcutaneously with injex in the mastectomised breast area.

Results:After hfPSC transplantation,we restored internal integrity of physiological breast tissue and its corresponding function regenerating arterial,venous,lymphatic,nerve,milk duct system,and papilla with lactogenic alveoli,lobules,lactiferous ducts,and galactophores which support colostrum responding to specific hormones.Breast cells were characterized by genomics,transcriptomics and proteomics of healthy breast cells.Simultaneously,with this method we have circumvented complications of embryonic stem cell use such as teratomas,and complication of reconstructive surgery such as capsular contracture,CTD,hematoma/seroma, calcification, atrophy, necrosis,fibromyalgia syndrome,neuroendocrine immunity,diffuse systemic inflammatory disease etc.

Conclusion:With autologous hfPSCs,we have achieved to restore complete anatomical and physiological integrity with functional viability of the entire mammary gland of mastectomised animal breast cancer models.